The cytotoxicity of maitotoxin to LLC-PK(1) and its antagonism to calcium channel blocking agents / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 22-24, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-295790
ABSTRACT
<p><b>OBJECTIVE</b>To study the cytotoxicity of maitotoxin (MTX) and its protective effects on calcium-channel blocking agents, so as to provide the data for control and treatment of MTX poisoning.</p><p><b>METHODS</b>Cytotoxicity was measured by MTT detecting system, and cytoplasmic free [Ca(2+)]i was measured by F-4500 fluorometry.</p><p><b>RESULTS</b>Incubation with 8 ng/ml MTX for 3 h reduced the survival ratio of LLC-PK(1) cells. The response was found in a time- and concentration-dependent manner, with significant differences as compared with the control group. The MTX-induced increase in [Ca(2+)]i was inhibited by Verapamil and Nifedipine at 5 x 10(-5) mol/L and 1 x 10(-4) mol/L respectively. Both of them significantly reduced the death of the LLC-PK(1) cells.</p><p><b>CONCLUSIONS</b>Cytotoxicity of MTX may be caused by the elevated intracellular [Ca(2+)]i. Calcium-channel blocking agents could protect LLC-PK(1) cells from injury by MTX.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Porcinos
/
Bloqueadores de los Canales de Calcio
/
Nifedipino
/
Verapamilo
/
Calcio
/
Células LLC-PK1
/
Oxocinas
/
Antagonismo de Drogas
/
Toxicidad
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Preventive Medicine
Año:
2002
Tipo del documento:
Artículo
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