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The cytotoxicity of maitotoxin to LLC-PK(1) and its antagonism to calcium channel blocking agents / 中华预防医学杂志
Chinese Journal of Preventive Medicine ; (12): 22-24, 2002.
Artículo en Chino | WPRIM | ID: wpr-295790
ABSTRACT
<p><b>OBJECTIVE</b>To study the cytotoxicity of maitotoxin (MTX) and its protective effects on calcium-channel blocking agents, so as to provide the data for control and treatment of MTX poisoning.</p><p><b>METHODS</b>Cytotoxicity was measured by MTT detecting system, and cytoplasmic free [Ca(2+)]i was measured by F-4500 fluorometry.</p><p><b>RESULTS</b>Incubation with 8 ng/ml MTX for 3 h reduced the survival ratio of LLC-PK(1) cells. The response was found in a time- and concentration-dependent manner, with significant differences as compared with the control group. The MTX-induced increase in [Ca(2+)]i was inhibited by Verapamil and Nifedipine at 5 x 10(-5) mol/L and 1 x 10(-4) mol/L respectively. Both of them significantly reduced the death of the LLC-PK(1) cells.</p><p><b>CONCLUSIONS</b>Cytotoxicity of MTX may be caused by the elevated intracellular [Ca(2+)]i. Calcium-channel blocking agents could protect LLC-PK(1) cells from injury by MTX.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Porcinos / Bloqueadores de los Canales de Calcio / Nifedipino / Verapamilo / Calcio / Células LLC-PK1 / Oxocinas / Antagonismo de Drogas / Toxicidad Límite: Animales Idioma: Chino Revista: Chinese Journal of Preventive Medicine Año: 2002 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Porcinos / Bloqueadores de los Canales de Calcio / Nifedipino / Verapamilo / Calcio / Células LLC-PK1 / Oxocinas / Antagonismo de Drogas / Toxicidad Límite: Animales Idioma: Chino Revista: Chinese Journal of Preventive Medicine Año: 2002 Tipo del documento: Artículo