Curcumin, a potential inhibitor of up-regulation of TNF-alpha and IL-6 induced by palmitate in 3T3-L1 adipocytes through NF-kappaB and JNK pathway / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 32-39, 2009.
Article
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| ID: wpr-296007
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the attenuating effect of curcumin, an anti-inflammatory compound derived from dietary spice turmeric (Curcuma longa) on the pro-inflammatory insulin-resistant state in 3T3-L1 adipocytes.</p><p><b>METHODS</b>Glucose uptake rate was determined with the [3H] 2-deoxyglucose uptake method. Expressions of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) were measured by quantitative RT-PCR analysis and ELISA. Nuclear transcription factor kappaB p65 (NF-kappa p65) and mitogen-activated protein kinase (MAPKs) were detected by Western blot assay.</p><p><b>RESULTS</b>The basal glucose uptake was not altered, and curcumin increased the insulin-stimulated glucose uptake in 3T3-L1 cells. Curcumin suppressed the transcription and secretion of TNF-alpha and IL-6 induced by palmitate in a concentration-dependent manner. Palmitate induced nuclear translocation of NF-kappaB. The activities of Jun NH2-terminal kinase (JNK), extracellular signal-regulated kinase1/2 (ERK1/2) and p38MAPK decreased in the presence of curcumin. Moreover, pretreatment with SP600125 (inhibitor of JNK) instead of PD98059 or SB203580 (inhibitor of ERK1/2 or p38MAPK, respectively) decreased the up-regulation of TNF-alpha induced by palmitate.</p><p><b>CONCLUSION</b>Curcumin reverses palmitate-induced insulin resistance state in 3T3-L1 adipocytes through the NF-kappaB and JNK pathway.</p>
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Índice:
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Asunto principal:
Palmitatos
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Farmacología
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Resistencia a la Insulina
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Regulación hacia Arriba
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Antiinflamatorios no Esteroideos
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FN-kappa B
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Interleucina-6
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Factor de Necrosis Tumoral alfa
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Sistema de Señalización de MAP Quinasas
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Curcumina
Límite:
Animals
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Año:
2009
Tipo del documento:
Article