In vitro antigenotoxicity of Ulva rigida C. Agardh (Chlorophyceae) extract against induction of chromosome aberration, sister chromatid exchange and micronuclei by mutagenic agent MMC / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 492-498, 2008.
Artículo
en Inglés
| WPRIM
| ID: wpr-296018
ABSTRACT
<p><b>OBJECTIVE</b>To determine the in vitro possible clastogenic and cytotoxic activities of Ulva rigida crude extracts (URE), and identify their antigenotoxic and protective effects on chemotherapeutic agent mitomycine-C (MMC).</p><p><b>METHODS</b>Anti-clastogenic and anti-genotoxic activities of Ulva rigida crude extracts (URE) were studied using chromosome aberration (CA), sister chromatid exchange (SCE), and micronuclei (MN) tests in human lymphocytes cultured in vitro.</p><p><b>RESULTS</b>The chromosome aberration, sister chromatid exchange or micronuclei tests showed that URE at concentrations of 10, 20, and 40 microg/mL had no clastogenic activity in human lymphocyte cell culture. Three doses of URE significantly decreased the number of chromosomal aberrations and the frequencies of SCE and MN when compared with the culture treated with MMC (P < 0.0001).</p><p><b>CONCLUSION</b>Although URE itself is not a clastogenic or cytotoxic substance, it possesses strong antigenotoxic, anti-clastogenic, and protective effects on MMC in vitro.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Intercambio de Cromátides Hermanas
/
Extractos Vegetales
/
Linfocitos
/
Pruebas de Micronúcleos
/
Células Cultivadas
/
Química
/
Aberraciones Cromosómicas
/
Antimutagênicos
/
Relación Dosis-Respuesta a Droga
Límite:
Humanos
Idioma:
Inglés
Revista:
Biomedical and Environmental Sciences
Año:
2008
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS