Generation of Japanese Encephalitis Virus-like Particle Vaccine and Preliminary Evaluation of Its Protective Efficiency / 病毒学报
Chinese Journal of Virology
;
(6): 150-155, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-296204
ABSTRACT
The cDNA fragment of JEV prME gene was cloned into the baculovirus shuttle vector (bacmid) to construct a recombinant baculovirus vector, defined as AcBac-prME. Then the recombinant baculovirus Ac-prME was obtained by transfecting Sf9 cells with AcBac-prME. Western blot analysis and immunofluorescence results indicated that both prM and E proteins were efficiently expressed in Sf9 cells. Electron microscopy suggested that prME was assembled into JEV-VLPs. To further evaluate the potential of JEV-VLPs as vaccine, the mice were immunized with JEV-VLPs and then challenged with lethal JEV. The results of mice survival and pathological changes demonstrated that the JEV-VLPs performed complete protection against JEV-P3 strain and relieved pathological changes in the mice brain significant. This study suggest that JEV-VLPs would be a potential vaccine for Japanese encephalitis virus.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Virología
/
Proteínas del Envoltorio Viral
/
Vacunación
/
Encefalitis Japonesa
/
Vacunas contra la Encefalitis Japonesa
/
Virus de la Encefalitis Japonesa (Especie)
/
Alergia e Inmunología
/
Vacunas de Partículas Similares a Virus
/
Células Sf9
/
Genética
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2016
Tipo del documento:
Artículo
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