Preparation and Identification of High Immunogenic A/PR/8/34 Maternal Strain HA Protein for Influenza Virus Classical Reassortment / 病毒学报
Chinese Journal of Virology
;
(6): 141-144, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-296206
ABSTRACT
Preparation of maternal strain A/PR/8/34 HA antiserum for influenza virus classical reassortment. A/PR/8/34 virus was digested by bromelain after inactivation and purification. 5%-20% sucrose continuous density gradient centrifugation method was used to purify HA protein. SIRD method was used to select the target protein. SDS-PAGE method was used to identified HA protein. High Immunogenic A/PR/8/34 HA protein was successfully prepared and HI titer reached 10240. High purity HA antiserum was identified by SIRD method. The key reagent in the classical reassortment of influenza virus was prepared, and the complete set of technical methods were explored, which laid the foundation for the independent research and development of seasonal influenza vaccine strains of China.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Virología
/
Pruebas de Inhibición de Hemaglutinación
/
Virus Reordenados
/
Glicoproteínas Hemaglutininas del Virus de la Influenza
/
Electroforesis en Gel de Poliacrilamida
/
Alergia e Inmunología
/
Gripe Humana
/
Subtipo H1N1 del Virus de la Influenza A
/
Genética
/
Anticuerpos Antivirales
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2016
Tipo del documento:
Artículo
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