Expression of Chimeric Influenza Hemagglutinin Antigen (cH7/3) using the Baculovirus Expression System and Identification of its Biological Activities / 病毒学报
Chinese Journal of Virology
;
(6): 524-529, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-296253
ABSTRACT
Hemagglutinin (HA) contains a head domain with a high degree of variability and a relatively conserved stem region. HA is the major viral antigen on the surface of the influenza virus. To define the biologic activities of chimeric HA bearing different head domains and stem regions or their potential use, a HA chimeric gene containing the head domain of the H7 subtype virus and stem region of the H3 subtype virus was modified and expressed using a baculovirus expression vector. Then, the secreted protein was purified and its biologic activities characterized. Approximately 1.4 mg/mL cH7/3 HA could be obtained, and its molecular weight was ≈ 70 kD. The trimer form of cH7/3 protein had hemagglutination activity and could be recognized by specific antibodies. The method described here can be used for further studies on the screening of HA stem-reactive antibodies or the development of vaccines with conserved epitopes.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Virología
/
Vacunas contra la Influenza
/
Expresión Génica
/
Baculoviridae
/
Glicoproteínas Hemaglutininas del Virus de la Influenza
/
Alergia e Inmunología
/
Gripe Humana
/
Vectores Genéticos
/
Genética
/
Hemaglutinación
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2015
Tipo del documento:
Artículo
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