Development of one step RT-PCR technique for detection of H7 subtype avian influenza / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1658-1663, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-296876
ABSTRACT
According to 45 hemagglutinin (HA) gene sequences of H7 subtype of avian influenza virus (AIV), a pair of specific oligonucleotide primers was designed. We developed one step RT-PCR for detecting AIV subtype H7. Sensitivity to detection of allantoic fluid by one step RT-PCR reached 10(5.5) EID50/mL and detection of swab samples reached 10(3) EID50/mL. We simultaneity detected the tissue and swab samples infected with H7 subtypes AIV by one step RT-PCR and virus isolation method. The results showed that the sensitivity of the assay gave an excellent correlation with the conventional virus isolation method. H1-H15 subtypes of avian influenza and other avian diseases were detected by the one step RT-PCR. The results showed the assays were high specific, without cross-reaction with other subtypes or other avian diseases. Development of one step RT-PCR will provide effective technical support for the rapid diagnosis and surveillance of molecular epidemiology of AIV subtype H7.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Virología
/
Aves
/
Sensibilidad y Especificidad
/
Clasificación
/
Glicoproteínas Hemaglutininas del Virus de la Influenza
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Subtipo H7N1 del Virus de la Influenza A
/
Gripe Aviar
/
Genética
/
Métodos
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2009
Tipo del documento:
Artículo
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