Construction and immunogenicity of recombinant adenoviruses expressing Cap protein of PCV2 and GP5 protein of PRRSV in mice / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1639-1645, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-296879
ABSTRACT
Porcine circovirus 2 (PCV2) has been implicated as the etiological agent of postweaning multisystemic wasting syndrome (PMWS). Co-infection of PCV2 and porcine reproductive and respiratory syndrome virus (PRRSV) can result in severe economic losses to the swine industry. In this study, we constructed the recombinant adenovirus rAd-Cap-GP5 expressing Cap of PCV2 and GP5 of PRRSV. And the expression of Cap and GP5 protein in the HEK-293 cells inoculated with rAd-Cap-GP5 were confirmed by immunoperoxidase monolayer assay (IPMA), indirect immunofluorescence assay (IFA) and Western blotting, respectively. The immunogenicity of recombinant adenoviruses rAd-Cap-GP5 was examined in mice by vaccination with the recombinant adenovirus. The results showed that the mice could produce anti-PCV2 and PRRSV antibodies detected by indirect ELISA and virus neutralization assay. It indicated that rAd-Cap-GP5 could provide humoral immunity responses in mice. The recombinant adenovirus rAd-Cap-GP5 might be an attractive candidate vaccine for preventing the disease associated with PCV2 and PRRSV infection.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Porcinos
/
Virología
/
Proteínas Recombinantes de Fusión
/
Transfección
/
Línea Celular
/
Adenoviridae
/
Proteínas del Envoltorio Viral
/
Inmunización
/
Circovirus
/
Infecciones por Circoviridae
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2009
Tipo del documento:
Artículo
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