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Patterning different cells based on microfluidics and self-assembled monolayers / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1234-1239, 2009.
Artículo en Chino | WPRIM | ID: wpr-296933
ABSTRACT
The ability to pattern multiple cells through precise surface engineering of cell culture substrates has promoted the development of cellular bioassays, such as differentiation, interaction and molecular signaling pathways. There are several well developed ways to pattern cells. This report describes a method for patterning multiple types of cells based on microfluidics and self-assembled monolayers. We developed two types of micro-dam structures by soft-lithography to locate cells precisely and modified the substrate by a kind of self-assembled monolayer with property of electrochemical desorption to confine cells in specific areas. Finally we could pattern an array of two different types of cells closely and precisely. Cells were confined in specific areas but still shared the same microenvironment, so they could interact through soluble molecules. The substrate was transparent and open, so we could easily apply several instruments for research. With these merits, this cell chip is appropriate for investigating the interaction between different types of cells.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Fisiología / Especificidad por Sustrato / Venas Umbilicales / Adhesión Celular / Células / Biología Celular / Ingeniería de Tejidos / Línea Celular Tumoral / Células Endoteliales Límite: Humanos Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2009 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Fisiología / Especificidad por Sustrato / Venas Umbilicales / Adhesión Celular / Células / Biología Celular / Ingeniería de Tejidos / Línea Celular Tumoral / Células Endoteliales Límite: Humanos Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2009 Tipo del documento: Artículo