A novel screening system for Pseudomonas aeruginosa quorum sensing inhibitors / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1173-1179, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-296941
ABSTRACT
Quorum sensing is an important gene regulatory mechanism in Pseudomonas aeruginosa and controls the expression of numerous virulence factors. We designed and constructed a screening system for quorum-sensing inhibitors. We developed the system by using the lasI and rhlA promoters fused with promoterless sacB as reporters. Using this system we screened a number of Chinese herb extracts, and identified three herb extracts containing inhibitors to the quorum-sensing system and to its regulated genes. The screening system developed was highly efficient and sensitive. It could serve as a useful tool to identify herb compounds that block infections but unlikely render antibiotic resistance in pathogens.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Pseudomonas aeruginosa
/
Medicamentos Herbarios Chinos
/
Química
/
Regiones Promotoras Genéticas
/
Genes Reporteros
/
Acanthaceae
/
Valerianaceae
/
Evaluación Preclínica de Medicamentos
/
Percepción de Quorum
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
/
Estudio de tamizaje
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2009
Tipo del documento:
Artículo
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