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The study on EPC originated from human umbilical cord blood promoting neovascularization in ischemic skin flap / 中华整形外科杂志
Chinese Journal of Plastic Surgery ; (6): 206-208, 2007.
Artículo en Chino | WPRIM | ID: wpr-297062
ABSTRACT
<p><b>OBJECTIVE</b>Injecting the EPC into the corresponding skin flap to study EPC biological characteristics and its effect on neovascularization in ischemia skin flap.</p><p><b>METHODS</b>CD133 + cells were enriched from human umbilical cord blood by immunomagnetic sorting, and cultured with EGM - 2MV media. After labeled with PKH26 (fluorescent cell linker), the EPC were injected into the over-length flap models made on athymic mice. Observing the EPCs trace and their participating in the flap vascularization using a fluorescent microscope. The potential of EPC neovascularization in ischemic tissue of skin flap was evaluated through measuring the necrotic area and vessel diameter and quantity in the skin flap.</p><p><b>RESULTS</b>The skin flap necrosis area of EPC group is significantly smaller than that of control (P < 0.05), the dermal and hypodermal blood perfusion of EPC group is significantly more than that of control (P < 0.05). Immunohistological and label fluorescent analyses showed vWF antigen-positive cells and labeled cells constructing blood vessels of flap.</p><p><b>CONCLUSIONS</b>Our data support the EPC may contribute to angiogenesis, speed up ischemic tissue vascularization.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Células Madre / Colgajos Quirúrgicos / Células Cultivadas / Biología Celular / Trasplante de Células Madre de Sangre del Cordón Umbilical / Sangre Fetal / Supervivencia de Injerto / Ratones Endogámicos BALB C / Ratones Desnudos Tipo de estudio: Estudio pronóstico Límite: Animales / Femenino / Humanos Idioma: Chino Revista: Chinese Journal of Plastic Surgery Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Células Madre / Colgajos Quirúrgicos / Células Cultivadas / Biología Celular / Trasplante de Células Madre de Sangre del Cordón Umbilical / Sangre Fetal / Supervivencia de Injerto / Ratones Endogámicos BALB C / Ratones Desnudos Tipo de estudio: Estudio pronóstico Límite: Animales / Femenino / Humanos Idioma: Chino Revista: Chinese Journal of Plastic Surgery Año: 2007 Tipo del documento: Artículo