An observation of the effects of the truncated septin2 on mouse epidermal cell and fibroblast / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 147-150, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-297072
ABSTRACT
<p><b>OBJECTIVE</b>To construct eukaryotic expression vector of the truncated septin2 and investigate the influence on the cultured mouse epidermal cell and fibroblast in vitro exerted by the transgenic expression product.</p><p><b>METHODS</b>The short splicing fragment was obtained by amplifying the reverse transcription product of the fetal mouse skin mRNA with PCR. Then its recombinant expression vector pcDNA3.1 (-)/septin2s was constructed and used to transfect the mouse epidermal cell and fibroblast cultured in vitro. The expression of the foreign gene was detected with RT-PCR and the changes of cell proliferation were observed and analysed.</p><p><b>RESULTS</b>RT-PCR results indicated that pcDNA3.1/septin2 was expressed in the cultured mouse epidermal cells and fibroblasts in vitro. We found that the epidermal cells accelerated their reproduction, but the fibroblasts had no obvious changes.</p><p><b>CONCLUSION</b>We successfully constructed eukaryotic expressive vectors of pcDNA3.1/ septin2s and transfected it into mouse epidermal cells and fibroblasts in vitro. The results settle a basis for showing effect of septin2s on fetal mouse skin.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Piel
/
ADN Recombinante
/
Transfección
/
Línea Celular
/
Proteínas de Unión al GTP
/
Biología Celular
/
Proteínas del Citoesqueleto
/
Células Epiteliales
/
Septinas
/
Fibroblastos
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Plastic Surgery
Año:
2007
Tipo del documento:
Artículo
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