Effect of jiedu quyu zishen recipe on TLR9 signal pathway of murine macrophage cells / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 466-470, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-297405
ABSTRACT
<p><b>OBJECTIVE</b>To explore efficacy enhancing and detoxification roles of Jiedu Quyu Zishen Recipe (JQZR) in treating systemic lupus erythematosus (SLE) by studying its effect on Toll like receptor 9 (TLR9) signal pathway of murine macrophage cells after JQZR stimulated CpG oligodeoxynucletide (CpG ODN).</p><p><b>METHODS</b>Murine macrophage cells in vitro cultured were randomly divided into 4 groups, i.e., the blank serum group, the CpG ODN stimulus group, the CpG ODN + dexamethasone group, the CpG ODN + medicated serum group. Murine macrophage cells were collected after 24-h intervention. The expression of TLR9, myeloid differentiation factor 88 (MyD88), NF-KB, IFN-α mRNA were analyzed by RT-PCR. The expression of TLR9 and NF-κB protein were analyzed by Western blot. Changes of the NF-KB transcriptional activity were assayed by Dual-Luciferase reporter assay system.</p><p><b>RESULTS</b>mRNA expressions of TLR9, MyD88, NF-κB, and IFN-α, protein expressions of TLR9 and NF-κB, and NF-κB transcriptional activities were enhanced, showing statistical difference when compared with those of the blank serum group (P <0. 05, P <0. 01). Compared with the CpG ODN stimulus group, mRNA expressions of MyD88, NF-κB, and IFN-α, the protein expression of NF-κB and the NF-κB transcriptional activities decreased in the CpG ODN + dexamethasone group with statistical difference (P <0. 01). Compared with the CpG ODN stimulus group, mRNA expressions of TLR9, MyD88, NF-κB, and IFN-α, protein expressions of TLR9 and NF-κB, and NF-κB transcriptional activities were decreased in CpG ODN+ medicated serum group with statistical difference (P <0. 01).</p><p><b>CONCLUSION</b>Efficacy enhancing and detoxification roles of JQZR in treatment of SLE might be realized through regulating TLR9 signal pathways.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
ARN Mensajero
/
Medicamentos Herbarios Chinos
/
Transducción de Señal
/
Línea Celular
/
FN-kappa B
/
Receptor Toll-Like 9
/
Factor 88 de Diferenciación Mieloide
/
Macrófagos
/
Metabolismo
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Año:
2015
Tipo del documento:
Artículo
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