MCPIP1 mediates MCP-1-induced vascular smooth muscle cell proliferation / 生理学报
Acta Physiologica Sinica
;
(6): 616-622, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-297530
ABSTRACT
The aim of the present study is to investigate whether monocyte chemotactic protein-1 (MCP-1)-induced vascular smooth muscle cell (VSMC) proliferation is mediated via monocyte chemotactic protein-1 induced protein-1 (MCPIP1). MCPIP1 expressions in cultured VSMC were detected by real-time PCR and Western blot following MCP-1 incubation. After MCPIP1 was silenced by siRNA, cell number was counted by hemocytometer, VSMC activity was analyzed by CCK-8 kit, percentage of DNA synthesis was detected by EdU kit, percentage of S phase cell numbers were measured by flow cytometry, and c-fos mRNA expression induced by MCP-1 or platelet-derived growth factor (PDGF) was detected by real-time PCR. The results showed MCP-1 increased MCPIP1 mRNA and up-regulated MCPIP1 protein expression in dose- and time-dependent manners. Cell counts, cellular activity, the percentage of DNA synthesis, and the percentage of S phase cell numbers were remarkably decreased in MCPIP1 siRNA group, compared with those in MCP-1 group. The enhancing effect of MCP-1 or PDGF on c-fos mRNA expression was inhibited by MCPIP1 siRNA. These results suggest that MCP-1-induced VSMC proliferation is mediated via MCPIP1, and the underlying mechanism may involve c-fos expression up-regulation.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Ribonucleasas
/
Factores de Transcripción
/
Factor de Crecimiento Derivado de Plaquetas
/
Regulación hacia Arriba
/
Células Cultivadas
/
Quimiocina CCL2
/
Biología Celular
/
Miocitos del Músculo Liso
/
Proliferación Celular
Límite:
Humanos
Idioma:
Chino
Revista:
Acta Physiologica Sinica
Año:
2013
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS