Differential proteomic expression in human liver cells stimulated by hydroquinone / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 658-661, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-297613
ABSTRACT
<p><b>OBJECTIVE</b>To explore the differential proteomic expression in human liver cells L-02 after exposure to HQ.</p><p><b>METHODS</b>Subcultured L-02 cells were treated by HQ for 24 h at a 1 x 10(-4) mol/L concentration and a blank group was set as the control. Immediately after the treatment, total cellular proteins were extracted and separated by 2-DE, and the images were analyzed by PDQuest software. The experiment was totally repeated 3 times with 3 repetitions for each group every time. The well repeated spots were identified by MALDI-TOF MS and then searched in NCBI human protein database with Mascot.</p><p><b>RESULTS</b>About 1,000 spots per gel were found. Compared with the control group, 17, 18 and 24 spots were significantly altered in 3 separate experiments. The 4 well repeated spots were identified by MALDI-TOF MS as Rho GDP dissociation inhibitor GDI alpha, 6-phosphogluconolactonase, erbB3 binding protein EBP1 and lamin A/C, isoform 1 precursor. They were involved in cell skeleton, signal transduction and energy metabolization in functional classification.</p><p><b>CONCLUSION</b>Hydroquinone can change the protein expression in liver cells, which provides clues for exploring the toxic mechanism.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Espectrometría de Masas
/
Electroforesis en Gel Bidimensional
/
Línea Celular
/
Reproducibilidad de los Resultados
/
Hepatocitos
/
Proteómica
/
Toxicidad
/
Hidroquinonas
/
Metabolismo
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Año:
2006
Tipo del documento:
Artículo
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