Bombesin increases cytokeratin expression and free calcium concentration in PC-3 cell line / 中华男科学杂志
National Journal of Andrology
;
(12): 332-334, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-297730
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of bombesin noncytoskeleton form and intracellular free calcium ([Ca2+]i) concentration in PC-3 prostate cancer cell line.</p><p><b>METHODS</b>Immunofluorescent histochemistry (IH) combined with laser scanning confocal microscopy (LSCM) was used to examine the expression of cytokeratin (CK) in PC-3 cells treated with definite concentrations of BBS and observe its effect on cytoskeleton form. Fluo-3/AM fluorescence technique and LSCM were adopted to measure the [Ca2+]i concentration after different concentrations (10(-9), 10(-7) and 10(-5) mol/L) of BBS were added in PC-3 cells.</p><p><b>RESULTS</b>BBS (10(-5) mol/L) stimulated the expression of CK in PC-3 cells and the formation of lamellipodium, and increased the [Ca2+]i concentration, with concentration dependence.</p><p><b>CONCLUSION</b>Definite concentrations of BBS could obviously enhance the [Ca2+] i concentration, CK expression and cytoskeleton morphology of PC-3 cells. The results provide a basis for further studies on the role of BBS in tumour researches as well as in intracellular signal transmission.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Neoplasias de la Próstata
/
Citoesqueleto
/
Albúmina Sérica Bovina
/
Bombesina
/
Células Tumorales Cultivadas
/
Fluoroinmunoensayo
/
Calcio
/
Microscopía Confocal
/
Queratinas
Límite:
Humanos
/
Masculino
Idioma:
Chino
Revista:
National Journal of Andrology
Año:
2007
Tipo del documento:
Artículo
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