Molecular basis of one-way serological reaction between SINV and XJ-160 virus / 病毒学报
Chinese Journal of Virology
;
(6): 228-233, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-297879
ABSTRACT
The purpose of this study is to elucidate the molecular mechanism of one-way serological reaction between XJ-160 virus and SINV by recombinant viruses which exchanged the glycoprotein genes individually or simultaneously. Three recombinant viruses were obtained based on the whole-length infectious cDNA clone of XJ-160 virus. The infectivity and pathogenesis to BHK-21 cells and animals were studied and the gene which controlled this one-way serological reaction phenomenon was searched by MCPENT. The results showed that the E2 glycoprotein was the main factor which influenced the growth rate, plaque morphology and pathogenicity of BHK-21 cells and suckling mice. The results of MCPENT showed that the E2 glycoprotein of SINV played a major role in this one-way serological reaction phenomenon. Our study identified the SINE2 gene was the determined gene for one way serological reaction between XJ-160 virus and SINV, and this research laid the foundation for further analysis of the genomic structure and function of SINV.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Virus Sindbis
/
Proteínas Virales
/
ADN Recombinante
/
Pruebas de Neutralización
/
Datos de Secuencia Molecular
/
Glicoproteínas
/
Ingeniería Genética
/
Línea Celular
/
Química
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2010
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS