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SiRNA targeting ICP4 attenuates HSV-1 replication / 病毒学报
Chinese Journal of Virology ; (6): 163-169, 2010.
Artículo en Chino | WPRIM | ID: wpr-297889
ABSTRACT
HSV-1, a neurotropic virus, always leads to severe nervous symptoms. It is hard to completely eradicate the latent viruses after conventional therapy so that recurrence is inevitable. ICP is a key regulator for HSV replication and transcription that determines the cytolytic infection or latent state. In search of new anti-virus strategy targeting HSV-1ICP4, two pairs of siRNA were designed, and a recombinant eukaryotic lentiviral expression plasmid pLKO-puro(r)-hU6-siRNA was constructed. Vero cells were transfected with the designed siRNAs by Lipofectamine 2000 and four stable monoclonal cell lines were established by puromycin screening method. The ICP4 expression at mRNA level was detected with real-time PCR, and the HSV-1 replication was measured with TCID50 assay. SiRNA was shown as an effective way to inhibit the expression of ICP4 in monoclonal cell lines. Meanwhile, HSV-1 replication was significantly inhibited when ICP4 was shut down by siRNA. We conclude that siRNA targeting ICP4 attenuates HSV-1 replication. Further more, multi-site siRNAs show stronger inhibitory effect on viral replication, which may be an effective and feasible approach for biological anti-viral drugs.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fisiología / Replicación Viral / Células Vero / ARN Mensajero / Secuencia de Bases / Terapia Genética / Chlorocebus aethiops / Proteínas Inmediatas-Precoces / Herpesvirus Humano 1 / ARN Interferente Pequeño Límite: Animales / Humanos Idioma: Chino Revista: Chinese Journal of Virology Año: 2010 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fisiología / Replicación Viral / Células Vero / ARN Mensajero / Secuencia de Bases / Terapia Genética / Chlorocebus aethiops / Proteínas Inmediatas-Precoces / Herpesvirus Humano 1 / ARN Interferente Pequeño Límite: Animales / Humanos Idioma: Chino Revista: Chinese Journal of Virology Año: 2010 Tipo del documento: Artículo