Antibody preparation and enzyme linked immunosorbent assay of Lonicera japonica phenylalnine ammonia lyase / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1498-1502, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-298052
ABSTRACT
The expression of phenylalnine ammonia lyase (LJPAL1) is closely related to the content of active compounds in Lonicera japonica. In this paper, a prokaryotic expression vector is constructed and LJPAL1 protein is expressed in E. coli. Three antigen sites were synthesized to peptide antigen and prepared polyclonal antibody of Anti-LJT-1, Anti-LJT-2 and Anti-LJT-3, separately. Antibody Anti-LJT-2 was screened using Western blotting. And indirect ELISA was built using Anti-LJT-2. The results of this study will be a base for honeysuckle chemical quality and evaluation kits.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fenilanina Amoníaco-Liasa
/
Plantas Medicinales
/
Plásmidos
/
Ensayo de Inmunoadsorción Enzimática
/
Química
/
Western Blotting
/
Estructura Terciaria de Proteína
/
Estructura Secundaria de Proteína
/
Regulación de la Expresión Génica de las Plantas
/
Lonicera
Idioma:
Chino
Revista:
Acta Pharmaceutica Sinica
Año:
2013
Tipo del documento:
Artículo
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