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Role of 5-HT2A-receptors coupled with superoxide anion in the medial area of nucleus retrofacialis / 南方医科大学学报
Journal of Southern Medical University ; (12): 296-299, 2007.
Artículo en Chino | WPRIM | ID: wpr-298182
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of 5-HT2A-receptors coupled with superoxide anion (O2-) on respiratory regulation signal transductionin passageway in the medial area of nucleus retrofacialis (mNRF).</p><p><b>METHODS</b>mNRF island was prepared from medullary slices of neonatal SD rats according to Johnson's method and transferred separately into 24-well culture plates with reagents according to protocol, followed by incubation for 60 min at 37 degrees C in a humidified incubator with 5% CO2. Absorbance of 100 microl supernatant was measured by spectrophotometry at 550 nm and the effect of 5-HT and 2,5-dimethoxy-4-iodoamphetamine hydrochloride (DOI, agonist of 5-HT2A-receptors) on O2- generation in the mNRF was observed, along with the inhibition of this effect by ketanserin (antagonist of 5-HT2A-receptors) and alpha-lipoic acid (alpha-LA, a antioxidant).</p><p><b>RESULTS</b>5-HT concentration-response curve demonstrated that absorbance peak occurred at 1 micromol/L without further increment with higher concentration. DOI concentration-response curve showed the absorbance peak at 20 micromol/L without further increment. 5-HT and DOI significantly increased the absorbance with comparable effects. Ketanserin and alpha-LA significantly decreased the absorbance generated by 5-HT and DOI.</p><p><b>CONCLUSION</b>Activation of 5-HT2A receptors results in obvious O2- production in mNRF, suggesting that 5-HT2A receptors regulate respiratory function in association with O2-.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fisiología / Centro Respiratorio / Bulbo Raquídeo / Ratas Sprague-Dawley / Superóxidos / Receptor de Serotonina 5-HT2A / Animales Recién Nacidos / Metabolismo Límite: Animales Idioma: Chino Revista: Journal of Southern Medical University Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fisiología / Centro Respiratorio / Bulbo Raquídeo / Ratas Sprague-Dawley / Superóxidos / Receptor de Serotonina 5-HT2A / Animales Recién Nacidos / Metabolismo Límite: Animales Idioma: Chino Revista: Journal of Southern Medical University Año: 2007 Tipo del documento: Artículo