Differentially expressed transcription factor-related genes in nasopharyngeal carcinoma and nasopharyngeal tissues / 南方医科大学学报
Journal of Southern Medical University
;
(12): 146-149, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-298221
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the gene expression profiles of transcription factor-related genes in nasopharyngeal carcinoma (NPC) tissues and normal nasopharyngeal tissues using a cDNA microarray membrane for exploring the regulatory mechanism of differential gene express in NPC tissues.</p><p><b>METHODS</b>The total RNAs from 24 NPC tissues and 24 pooled normal nasopharyngeal tissues were reverse transcribed and labeled with alpha-(32)P-dCTP. The resultant cDNAs were hybridized to GF211 microarray, and the signals were analyzed by Pathway 4.0 software. RT-PCR was carried out to confirm the results.</p><p><b>RESULTS</b>Among the 1625 differentially expressed genes detected in NPC and nasopharyngeal tissues, 35 transcription factor-related genes were identified with either up- or down-regulation.</p><p><b>CONCLUSION</b>These differentially expressed transcription factor-related genes in NPC tissues might play a role in the regulation of NPC-related gene expression.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Factores de Transcripción
/
Proteínas Nucleares
/
Células Tumorales Cultivadas
/
Carcinoma de Células Escamosas
/
Regulación Neoplásica de la Expresión Génica
/
Neoplasias Nasofaríngeas
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Perfilación de la Expresión Génica
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2007
Tipo del documento:
Artículo
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