Cytotoxicity evaluation of biodegradable polylactic acid-chitin plates in osteosynthesis in vitro / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the cytotoxicity of polylactic acid-chitin plates in vitro.</p><p><b>METHODS</b>The cytotoxicity of polylactic acid-chitin plates was evaluated with MTT assay and direct contact assay using the cell line L929 in vitro. L929 cells were cultured with different concentrations of the extracts of polylactic acid-chitin plates (0, 50% and 100%, V/V) in vitro, and MTT assay was performed to evaluate the cell proliferation at 2, 4 and 7 days. The morphologic changes of the cells were observed by inverted microscope and scanning electron microscope.</p><p><b>RESULTS</b>MTT assay revealed that the extracts of polylactic acid-chitin plates enhanced the cell proliferation (P<0.01), but not in a dose-dependent manner (P>0.05). In the direct contact assay, no side effects were found in regard to the cell growth, proliferation and attachment to the polylactic acid-chitin plates as compared with the negative control. The cytotoxicity grade of polylactic acid-chitin plates was 0 or 1, suggesting that the material was almost free of cytotoxicity.</p><p><b>CONCLUSION</b>The polylactic acid-chitin plate possesses ideal cellular compatibility, and therefore has great potential for clinical use as an internal bone fixation material.</p>