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Effect of superparamagnetic iron oxide labeling on neural stem cell survival and proliferation / 南方医科大学学报
Journal of Southern Medical University ; (12): 49-55, 2007.
Artículo en Chino | WPRIM | ID: wpr-298246
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of superparamgnetic iron oxides (ferumoxides) on the survival and proliferation of neural stem cells (NSCs) and determine the optimal ferumoxides concentration for labeling.</p><p><b>METHODS</b>Bone marrow stromal cells (BMSCs) were obtained from rat femoral marrow and cultured in vitro to induce their differentiation into NSCs. Ferumoxides labeling of the NSCs was performed with different final concentrations of ferumoxides, and the labeling efficiency and viability of the labeled NSCs were evaluated by Prussian blue staining, MTT assay, flow cytometry and transmission electron microscope.</p><p><b>RESULTS</b>The NSCs could be effectively labeled with ferumoxides with a labeling efficiency of around 90%. Prussian blue staining showed numerous fine granules with blue staining in the cytoplasm of the labeled NSCs, and the intensity of the blue staining was in positive correlation with the ferumoxide concentration for labeling. Transmission electron microscopy of the labeled NSCs revealed the presence of numerous vesicles spreading in the cytoplasm and filled with electron-dense magnetic iron particles. The ferumoxides vesicles increased with the labeling concentration of ferumoxides, and at the final concentration exceeding 25 microg/ml, ferumoxides vesicles in the NSCs gave rise to conglomeration which hampered observation of the cellular ultrastructure by transmission electron microscope. The results of flow cytometry and MTT assay demonstrated that the cell viability, proliferation, differentiation and apoptosis of the labeled cells were affected by ferumoxides at the concentration above 25 microg/ml, but such effects could be minimal at lower concentrations.</p><p><b>CONCLUSION</b>Ferumoxides might be feasible for in vitro labeling of the NSCs with the optimal concentration of 25 microg/ml.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Óxidos / Farmacología / Células Madre / Supervivencia Celular / Células Cultivadas / Dextranos / Biología Celular / Microscopía Electrónica de Transmisión / Proliferación Celular / Óxido Ferrosoférrico Límite: Animales Idioma: Chino Revista: Journal of Southern Medical University Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Óxidos / Farmacología / Células Madre / Supervivencia Celular / Células Cultivadas / Dextranos / Biología Celular / Microscopía Electrónica de Transmisión / Proliferación Celular / Óxido Ferrosoférrico Límite: Animales Idioma: Chino Revista: Journal of Southern Medical University Año: 2007 Tipo del documento: Artículo