Alpha-glucosidase inhibitors from Luculia pinciana / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To search alpha-glucosidase inhibitors from Luculia pinciana.</p><p><b>METHOD</b>The alpha-glucosidase inhibitor was isolated by the column chromatographic techniques and the bioassay-guided method in vitro. A combination of MS and NMR spectroscopy was used to identify the chemical structures. The inhibitory kinetic of the isolations was also investigated.</p><p><b>RESULT</b>The ethyl acetate extract showed strongest inhibitory activity, and five active compounds were isolated and identified as scopletin (1), 5-methoxy-8-hydroxycoumarin (2), lalpha,3beta,24-trihydroxyursolic acid (3), ursolic acid (4) and oleanolic acid (5). The IC50 values of all compounds were lower than that of acarbose. Four of them shown noncompetitive type manner on alpha-glucosidase inhibition except that compound 3 is competitive type manner.</p><p><b>CONCLUSION</b>Compounds 1-4 as the inhibitors of alpha-glucosidase were reported for the first time.</p>