Differential Expression of Nerve Injury-Induced Protein 1 (Ninjurin 1) in In Vivo and In Vitro Models for Diabetic Erectile Dysfunction
Korean Journal of Urology
;
: 636-642, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-29843
ABSTRACT
PURPOSE:
Endothelial dysfunction and peripheral neuropathy are important mechanisms responsible for diabetes-induced erectile dysfunction (ED). Nerve injury-induced protein 1 (Ninjurin 1) is known to be related to neuroinflammatory processes and is also reported to induce vascular regression during the developmental period. In the present study, we determined the differential expression of Ninjurin 1 in penile tissue of streptozotocin (STZ)-induced diabetic mice with ED. MATERIALS ANDMETHODS:
Diabetes was induced in 8-week-old C57BL/6J mice by intraperitoneal injections of STZ (50 mg/kg for 5 days). Eight weeks later, erectile function was measured by electrical stimulation of the cavernous nerve (n=6 per group). The penis was then harvested for immunohistochemical analysis and Western blot analysis for Ninjurin 1 (n=4 per group). We also determined Ninjurin 1 expression in primary cultured mouse cavernous endothelial cells (MCECs) incubated under the following conditions normal glucose condition (5 mM), high-glucose condition (30 mM), and high-glucose condition (30 mM)+insulin (1 nM).RESULTS:
The expression of Ninjurin 1 protein was significantly higher in both cavernous endothelial cells and the dorsal nerve bundle of diabetic mice than in those of controls. In the in vitro study in MCECs, Ninjurin 1 expression was also significantly increased by the high-glucose condition and was returned to baseline levels by treatment with insulin.CONCLUSIONS:
Regarding the role of Ninjurin 1 in neuropathy and vascular regression, it would be interesting to examine the effects of inhibition of Ninjurin 1 on erectile function in animal models of ED with a vascular or neurogenic cause.
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Pene
/
Western Blotting
/
Estreptozocina
/
Enfermedades del Sistema Nervioso Periférico
/
Modelos Animales
/
Células Endoteliales
/
Diabetes Mellitus
/
Estimulación Eléctrica
/
Endotelio
/
Cuevas
Límite:
Animales
Idioma:
Inglés
Revista:
Korean Journal of Urology
Año:
2012
Tipo del documento:
Artículo
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