Expression and relationship of p27(kip1) and its related molecules Jab1 and CRM1 during proliferation of lymphoma cells U937 / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 657-661, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-298524
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression and relationship of p27(kip1) and its related molecules Jab1 and CRM1 during proliferation of lymphoma cells U937.</p><p><b>METHODS</b>U937 cells were treated with serum starvation and release, and the effects of these treatments on the cell growth was tested with cell number counting. The expression and localization of p27(kip1), Jab1 and CRM1 in U937 cells were detected by Western blot, double immunolabelling and laser scanning confocal microscopy.</p><p><b>RESULTS</b>The growth of U937 cells was blocked by serum starvation. The total protein of p27(kip1) was increased while Ser10-phosphorylated p27(kip1) -related molecules Jab1 and CRM1 were decreased. Meanwhile, the location of p27(kip1) was changed from cytoplasm into nuclei. After serum release, the location of p27(kip1) expression reappeared in the cytoplasm again.</p><p><b>CONCLUSION</b>During the proliferation process of lymphoma U937 cells, Jab1 and CRM1 may influence the location and expression of p27kip1, and may participate in regulation of growth of NHL cells.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Péptido Hidrolasas
/
Farmacología
/
Núcleo Celular
/
Medio de Cultivo Libre de Suero
/
Receptores Citoplasmáticos y Nucleares
/
Técnicas de Cultivo de Célula
/
Células U937
/
Citoplasma
/
Carioferinas
/
Péptidos y Proteínas de Señalización Intracelular
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Oncology
Año:
2007
Tipo del documento:
Artículo
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