FGF8 is Essential for Functionality of Induced Neural Precursor Cell-derived Dopaminergic Neurons
International Journal of Stem Cells
;
: 228-234, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-29876
ABSTRACT
Induced neural precursor cells (iNPCs) are one source of transplantable dopaminergic neurons used in cell therapy for Parkinson's disease. In the present study, we demonstrate that iNPCs can be generated by transducing Brn2, Ascl1, Myt1L and Bcl-xL in a culture supplemented with several mitogens and subsequently can be differentiated to dopaminergic neurons (DA). However, studies have shown that iDA and/or iNPC-derived DA neurons using various conversion protocols have low efficiency. Here, we show that early exposure of FGF8 to fibroblasts efficiently improves differentiation of DA neurons. So our study demonstrates that FGF8 is a critical factor for generation of iNPC-derived DA neurons.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Enfermedad de Parkinson
/
Neuronas Dopaminérgicas
/
Fibroblastos
/
Tratamiento Basado en Trasplante de Células y Tejidos
/
Mitógenos
/
Neuronas
Tipo de estudio:
Guía de Práctica Clínica
Idioma:
Inglés
Revista:
International Journal of Stem Cells
Año:
2015
Tipo del documento:
Artículo
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