Effects of vitamin D analogue EB1089 on proliferation and apoptosis of hepatic carcinoma cells / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 415-418, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-299213
ABSTRACT
<p><b>OBJECTIVE</b>This study aimed at investigating the effects of vitamin D analogue EB1089 on the proliferation and apoptosis of hepatic carcinoma cells.</p><p><b>METHODS</b>Hepatic carcinoma cell strain G(2) (Hep-G(2)) in which prominent vitamin D receptor (VDR) mRNA could be expressed and the cell strain T (HCC-T) negative in VDR gene expression were incubated in culture media with 100 nmol/L, 10 nmol/L and 1 nmol/L EB1089 for 2 d, 4 d and 6 d, respectively. Survival and proliferation of the cells were detected by blue tetrazolium colorimetric test and plate clone-forming test, the VDR mRNA expression was determined by reverse transcription-polymerase chain reaction (RT-PCR) and apoptosis of the cells was detected by flow cytometry (FCM) and electron microscopy.</p><p><b>RESULTS</b>EB1089 could inhibit the proliferation of hepatocellular cell line Hep-G(2) that expressed prominent vitamin D receptor mRNA, the inhibitory rate is 17.5% approximately 72.1%. On the other hand, EB1089 had no anti-proliferative effect on hepatocellular cell line HCC-T in which the gene expression of vitamin D receptors was negative. The electron microscope results showed that EB1089 could induce apoptosis of hepatocarcinoma cells and the percentages of apoptotic cells measured by flow cytometer was 21.4%. Cell cycle progression was blocked at G(1) phase with EB1089.</p><p><b>CONCLUSION</b>EB1089 could inhibit proliferation of human Hep-G(2), probably through VDR, and induce apoptosis of the cells.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Calcitriol
/
Células Tumorales Cultivadas
/
División Celular
/
Apoptosis
/
Carcinoma Hepatocelular
/
Neoplasias Hepáticas
/
Antineoplásicos
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Preventive Medicine
Año:
2004
Tipo del documento:
Artículo
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