Quantitative determination of 5 active ingredients in different harvest periods of Ligusticum chuanxiong by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1650-1655, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-300212
ABSTRACT
A simple and quick method is described for the determination of ferulic acid, senkyunolide I, senkyunolide H, senkyunolide A and ligustilide in rhizomes of Ligusticum chuanxiong. The 5 active ingredients in the sample was extracted using 40% ethanol and analyzed by reversed-phase high performance liquid chromatography (HPLC). Chromatography separation was performed using Agilent 1100 series HPLC system with a Symmetry C18 column and gradient elution with a mixture of three solvents solvent A, acetonitrile, solvent B, methanol and solvent C, 1% aqueous acetic acid, 0 min to 5 min A B C 20 40 40, 5 min to 30 min A B C 60 to 100 0 40 to 0. The effluent was monitored using a VWD detector set at 321 nm (0-4.3 min) and 275 nm (4.31-30 min). The flow rate was set at 1 mL x min(-1) and the injection volume was 10 microL. The column temperature was maintained at 35 degrees C. The calibration curve was linear (r > or = 0.99) over the tested ranges. The average recovery was 94.44%-103.1% (n = 6). The method has been successfully applied to the analysis in different harvest periods of L. chuanxiong samples. In this paper, single-factor randomized block design to study the 5 components content of L. chuanxiong on ten collecting stages. For the L. chuanxiong collected from April 15th to May 30rd, the content of 5 ingredients increased primarily, and then decreased. Determine the appropriate harvest time has important significance to the promotion of the quality of L. chuanxiong.
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Acetonitrilos
/
Solventes
/
Factores de Tiempo
/
Benzofuranos
/
4-Butirolactona
/
Química
/
Cromatografía Líquida de Alta Presión
/
Ácido Acético
/
Ácidos Cumáricos
/
Ligusticum
Tipo de estudio:
Ensayo Clínico Controlado
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2014
Tipo del documento:
Artículo
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