Mechanisms of p16 gene inactivation salivary adenoid cystic carcinoma / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the mechanism of p16 gene inactivation in salivary adenoid cystic carcinoma.</p><p><b>METHODS</b>53 cases of freshly excised salivary adenoid cystic carcinomas were studied. Polymerase chain reaction (PCR) and single-stranded conformation polymorphism analysis of polymerase chain reaction products (PCR-SSCP) were used to detect deletion and mutation of p16 gene in salivary adenoid cystic carcinomas. Methylation specific PCR (MSP) was used to detect the methylation status of p16 gene.</p><p><b>RESULTS</b>The homozygous deletion, mutation and hypermethylation of p16 gene were noted in 16 cases (30.2%), 4 cases (7.5%) and 26 cases (49.1%) respectively in 53 cases of salivary adenoid cystic carcinomas.</p><p><b>CONCLUSION</b>The main inactivation mechanisms of p16 gene in salivary adenoid cystic carcinoma were hypermethylation and homozygous deletion. The mutation p16 gene was rare in salivary adenoid cystic carcinoma.</p>