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Genic and non-genic regulation of low frequence pulsed electromagnetic fields on osteoblasts differentiation / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences ; (6): 568-574, 2016.
Artículo en Chino | WPRIM | ID: wpr-300846
ABSTRACT
To study the genic and non-genic regulation of 50 Hz 0.6 mT pulsed electromagnenic fields (PEMF) on rat calvarial osteoblasts (ROB) differentiation.ROBs were achieved by enzyme digestion, and treated with 50 Hz 0.6 mT PEMFs for 1.5 hours after subculture. The alkaline phosphatase (ALP) activity, mRNA transcription of ALP, Runx2 and OSX and protein expression of Runx2 and OSX were detected at 0, 3, 6, 9 and 12 hours after PEMF treatment.The ALP activity at 3 hours after treatment was significantly higher than that in the control(<0.01), while the mRNA transcription of ALP began to increase at 6 hours after treatment. The mRNA transcription of Runx2 increased immediately after treatment and regressed at 6 hours, then increased again. The protein expression of it corresponded but with a little lag. The mRNA transcription of OSX also raised instantly after treatment, then returned to the level of control at 6 hours, and lower than control at 12 hours significantly. The protein expression of it also corresponded but with a bit delay.There are genic regulation for the protein expression of Runx2 and OSX, and non-genic regulation for the ALP activity on the process of 50 Hz 0.6 mT PEMFs prompts ROBs differentiation.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Osteoblastos / Osteogénesis / Efectos de la Radiación / Factores de Transcripción / Diferenciación Celular / Células Cultivadas / Química / Campos Electromagnéticos / Fosfatasa Alcalina / Subunidad alfa 1 del Factor de Unión al Sitio Principal Límite: Animales Idioma: Chino Revista: Journal of Zhejiang University. Medical sciences Año: 2016 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Osteoblastos / Osteogénesis / Efectos de la Radiación / Factores de Transcripción / Diferenciación Celular / Células Cultivadas / Química / Campos Electromagnéticos / Fosfatasa Alcalina / Subunidad alfa 1 del Factor de Unión al Sitio Principal Límite: Animales Idioma: Chino Revista: Journal of Zhejiang University. Medical sciences Año: 2016 Tipo del documento: Artículo