Influence of selenium induced oxidative stress on spermatogenesis and lactate dehydrogenase-X in mice testis / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 227-232, 2004.
Artículo
en Inglés
| WPRIM
| ID: wpr-300847
ABSTRACT
<p><b>AIM</b>To evaluate the effect of oxidative stress on the spermatogenesis and lactate dehydrogenase-X (LDH-X) activity in mouse testis.</p><p><b>METHODS</b>For creating different levels of oxidative stress in mice, three selenium (Se) level diets were fed in separate groups for 8 weeks. Group 1 animals were fed yeast-based Se-deficient (0.02 ppm) diet. Group 2 and Group 3 animals were fed with the same diet supplemented with 0.2 ppm and 1 ppm Se as sodium selenite, respectively. After 8 weeks, biochemical and histopathological observations of the testis were carried out. LDH-X levels in the testis were analyzed by western immunoblot and ELISA.</p><p><b>RESULTS</b>A significant decrease in testis Se level was observed in Group 1 animals, whereas it was enhanced in Group 3 as compared to Group 2. The glutathione peroxidase (GSH-Px) activity was significantly reduced in both the liver and testis in Group 1, but not in Group 2 and 3. A significant increase in the testis glutathione-S-transferase (GST) activity was observed in Group 1, whereas no significant change was seen in Groups 2 and 3. Histological analysis of testis revealed a normal structure in Group 2. A significant decrease in the germ cell population in Group 1 was observed as compared to Group 2 with the spermatids and mature sperm affected the most. Decrease in the lumen size was also observed. In the Se-excess group (Group 3), displacement of germ cell population was observed. Further, a decrease in the LDH-X level in testis was observed in Group 1.</p><p><b>CONCLUSION</b>Excessive oxidative stress in the Se deficient group, as indicated by changes in the GSH-Px/GST activity, affects the spermatogenic process with a reduction in mature sperm and in turn the LDH-X level.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Fisiología
/
Selenio
/
Espermatogénesis
/
Testículo
/
Farmacocinética
/
Estrés Oxidativo
/
Dieta
/
Glutatión Transferasa
Límite:
Animales
Idioma:
Inglés
Revista:
Asian Journal of Andrology
Año:
2004
Tipo del documento:
Artículo
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