The effect of high frequency stimulation of epileptic foci on the release of glutamate and gamma-aminobutyric acid in hippocampus of the kainic acid-kindled rats / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 88-92, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-301494
ABSTRACT
<p><b>OBJECTIVE</b>To observe the dynamics of hippocampal release of glutamate (Glu) and gamma-aminobutyric acid (GABA) in epilepsy (TLE) after administration with high frequency stimulation (HFS).</p><p><b>METHODS</b>The SD were divided into four groups (n =10) (1) Control group (KB) the rats were injected intraperitoneally with saline 0.9%. (2) Kainic acid (KA) group the rats were injected with KA. (3) Pseudo-deep brain stimulation (DBS) group the KA-induced rats were implanted with rheophores alone. (4) DBS group KA induced-rats with DBS in hippocampal epileptic foci. We then collected hippocampal extracellular fluid by microdialysis and the levels of Glu and GABA were measured by high-performance liquid chromatography (HPLC) and fluorescence detection.</p><p><b>RESULTS</b>There was no difference in the baseline of Glu and GABA in the four groups. In contrast, a significant increase in the content of Glu and GABA was shown in the three periods of KA-kindled seizures. Electrical stimulation of hippocampus resulted in a decrease of hippocampal Glu contents, while there was no change in GABA contents. Additionally, HFS of hippocampus normalized the Glu/GABA ratio in the chronic period of seizures.</p><p><b>CONCLUSION</b>The high frequency stimulation of epileptic foci may protect against seizures by modulating the extracellular release of hippocampal Glu.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Terapéutica
/
Ratas Sprague-Dawley
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Ácido Glutámico
/
Secreciones Corporales
/
Estimulación Eléctrica
/
Epilepsia
/
Ácido gamma-Aminobutírico
/
Hipocampo
/
Ácido Kaínico
/
Excitación Neurológica
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2011
Tipo del documento:
Artículo
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