Study on leptin enhancing collagen systhesis in wounded rats / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 72-74, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-301498
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of leptin on collagen systhesis in wounded rats.</p><p><b>METHODS</b>Thirty male Wistar rats, weight (180 +/- 20)g, were randomly divided into three groups (n = 10) by weight normal depilation group, wound control group and leptin treatment group and ten rats were included in each group. A full-thickness defect measuring 2 x 2.5 cm was made in the back of rats in wound control group and leptin treatment group. Each wound in rats of leptin treatment group was applied topically with 0.1 ml leptin solution (2.0 microg leptin), daily for 7 days and that of wound control group with equivalent saline solution. All rats were killed and then granulation tissues samples and skin were collected to examine the synthesis of collagen.</p><p><b>RESULTS</b>Hydroxyproline content in granulation tissues of in leptin treatment group (33.92 +/- 3.09) mg/g were significantly increased than those in control group (29.55 +/- 3.59 mg/g, P < 0.05). The mRNA expressions of collagen I and III were significantly enhanced in leptin treatment group (0.96 +/- 0.09, 0.09 +/- 0.06) than those in control group (0.80 +/- 0.03, 0.08 +/- 0.03). The levels of type I and III collagen were significantly increased in leptin treatment group than those in control group.</p><p><b>CONCLUSION</b>Leptin applied topically can accelerate wound healing through enhancing gene expression of type I and III collagen and synthesis of collagen in wound tissue.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Cicatrización de Heridas
/
Heridas y Lesiones
/
Administración Cutánea
/
ARN Mensajero
/
Ratas Wistar
/
Leptina
/
Colágeno Tipo I
/
Colágeno Tipo III
/
Usos Terapéuticos
/
Quimioterapia
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2011
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS