Preparation of clenbuterol monoclonal antibody with subtractive immunization method / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 573-576, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-301558
ABSTRACT
<p><b>AIM</b>To obtain Clenbuterol monoclonal antibodies.</p><p><b>METHODS</b>Clenbuterol complete antigen was prepared with diazotization method. BALB/c mice was immunized with subtractive immunization, Clenbuterol monoclonal antibody was prepared with rule hybridoma technique.</p><p><b>RESULTS</b>The mice obtained tolerance to BSA by subtractive immunization. The rate of the hybridoma cell with positive reaction which had obtained was 8.2%, and the specific clenbuterol monoclonal antibody was obtained at last.</p><p><b>CONCLUSION</b>Monoclonal antibodies to micromolecule contaminant be prepared by subtractive immunization, could decrease the workload in the bolting of monoclonal antibodies, and increase the chance to obtain the antibody of expected.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Albúmina Sérica Bovina
/
Inmunización
/
Clenbuterol
/
Alergia e Inmunología
/
Hibridomas
/
Metabolismo
/
Métodos
/
Ratones Endogámicos BALB C
/
Anticuerpos Monoclonales
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2009
Tipo del documento:
Artículo
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