Effects of Rb1 and Rg1 on the expression of Bcl-2, Bax in apoptosis of HK-2 cells induced by the serum of kidney ischemia/reperfusion / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 496-499, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-301577
ABSTRACT
<p><b>AIM</b>To explore effects of Ginsenosides (Rb1, Rg1) on the expression of Bcl-2, Bax in the serum of kidney ischemia/reperfusion inducing apoptosis of HK-2 cells.</p><p><b>METHODS</b>The serum of rabbits with renal ischemia/reperfusion (SIR) and the control serum of rabbits (SC) were acquired and cultured with HK-2 cells. Detected apoptosis with TUNEL assay. The experiment was designed as control group,ischemia/ reperfusion group, Rb1 blocking group and Rg1 blocking group. To detect the expression of Bcl-2, Bax with immunocytochemistry after 24 hours' cultured.</p><p><b>RESULTS</b>The expression of Bax in Rb1 blocking group and Rg1 blocking group were significantly decreased (P < 0.01), the ratios of Bcl-2/Bax were increased as compared with ischemia/reperfusion group.</p><p><b>CONCLUSION</b>Rb1 and Rg1 have protective effects on apoptosis of HK-2 cells induced by serum of kidney ischemia/reperfusion.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Fisiología
/
Sangre
/
Daño por Reperfusión
/
Línea Celular
/
Apoptosis
/
Proteínas Proto-Oncogénicas c-bcl-2
/
Biología Celular
/
Ginsenósidos
/
Suero
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2009
Tipo del documento:
Artículo
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