Validation of the libraries of the serial analysis of gene expression by the application of real-time quantitative polymerase chain reaction / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 51-54, 2010.
Article
en Zh
| WPRIM
| ID: wpr-301595
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To validate and supplement the libraries of serial analysis of gene expression (SAGE) by the application of the real-time quantitative polymerase chain reaction PCR).</p><p><b>METHODS</b>The primers were designed based on the full sequences of the genes. Nine single matched tags, 6 multiple matched tags, 1 non-matched tag due to the update of the National Center for Biotechnology Information (NCBI) database, and 2 non-matched tags were selected to fulfill the validation of real-time PCR.</p><p><b>RESULTS</b>The genes were all specifically amplified by the primers pairs. The expressions of the single matched tags were identical to those of the SAGE libraries; however, the expressions of only 3 genes of the 6 multi-matched tags were identical to those of the SAGE libraries. The PCR data of the non-matched tag due to the update of the NCBI database were opposite to those of the SAGE libraries. The data did not support the significant difference of the non-matched gene of the SAGE libraries.</p><p><b>CONCLUSIONS</b>Real-time PCR is a reliable tool for the validation of high through-put data such as SAGE. The reliability of data depends on the match of the tags of the SAGE libraries.</p>
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Asunto principal:
Análisis de Secuencia por Matrices de Oligonucleótidos
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Perfilación de la Expresión Génica
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Reacción en Cadena en Tiempo Real de la Polimerasa
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Métodos
Idioma:
Zh
Revista:
Acta Academiae Medicinae Sinicae
Año:
2010
Tipo del documento:
Article