ShcD interacts with TrkC through its PTB and SH2 domains / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 720-723, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-301620
ABSTRACT
<p><b>OBJECTIVE</b>To study the interaction between ShcD and TrkC and to reveal the molecular mechanism of the downstream signal transduction of TrkC.</p><p><b>METHODS</b>Yeast two-hybrid assay was used. The intracellular domains of TrkC and TrkC mutants were cloned into pAS2-1, and ShcD and its four domains (CH2, PTB, CH1, and SH2 domains) were cloned into pACT2 vector respectively. The constructs were separately cotransformed into yeast. beta-galactosidase activity was measured to detect their interactions. TrkC was cloned into pmRFP (carrying red fluorescent protein), and ShcD was cloned into pEGFP (carrying green fluorescent protein). pmRFP-TrkC and pEGFP-ShcD were co-transfected into 293T cells, and then the cells were fixed and subjected to confocal analysis to study their subcellular localization.</p><p><b>RESULTS</b>ShcD interacted with TrkC but not with kinase dead mutant TrkCM1(K572A). Both PTB and SH2 domains were capable of binding to TrkC, and PTB domain bound NPQY motif of TrkC. ShcD colocalized with TrkC throughout the cytoplasm and in the plasma membrane in 293T cells.</p><p><b>CONCLUSION</b>ShcD binds to TrkC in a kinase-activity-dependent manner through its PTB and SH2 domains.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plásmidos
/
Unión Proteica
/
Transformación Bacteriana
/
Sitios de Unión
/
Transfección
/
Células Cultivadas
/
Dominios Homologos src
/
Receptor trkC
/
Técnicas del Sistema de Dos Híbridos
/
Proteínas Adaptadoras Transductoras de Señales
Límite:
Humanos
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2009
Tipo del documento:
Artículo
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