Relationship between cytochrome c-mediated caspase-3 activity and chemoresistance in cisplatin-resistant human ovarian cancer cell lines / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 544-547, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-301940
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of anti-apoptosis gene (bcl-X(L)), cytochrome c and caspase-3 activity on chemoresistance in cisplatin-resistant human ovarian cancer cell lines (A2780/DDP, COC1/DDP).</p><p><b>METHODS</b>The expression of bcl-X(L) cisplatin treated cytochrome c and caspase-3 activity were monitored by RT-PCR and Western blot in cisplatin-resistant (A2780/DDP, COC1/DDP) and cisplatin-sensitive (A2780, COC1) cell lines. The apoptotic rates of A2780, COC1, A2780/DDP and COC1/DDP were detected with flow cytometry after having been treated by cisplatin.</p><p><b>RESULTS</b>The expression of bcl-X(L) in A2780/DDP and COC1/DDP was significantly higher than that in A2780 and COC1 cells, whereas the expression of cytochrome c, caspase-3 activity and apoptotic rates of A2780/DDP and COC1/DDP were significantly reduced more than those of A2780 and COC1 after having been treated by cisplatin (P < 0.05).</p><p><b>CONCLUSION</b>The overexpression of anti-apoptotic gene bcl-X(L), which downregulates cytochrome c and decreases caspase-3 activity, may be related to cisplatin-resistance in human ovarian cancer cell lines.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Neoplasias Ováricas
/
Patología
/
Farmacología
/
Fisiología
/
Células Tumorales Cultivadas
/
Cisplatino
/
Apoptosis
/
Resistencia a Múltiples Medicamentos
/
Resistencia a Antineoplásicos
/
Proteínas Proto-Oncogénicas c-bcl-2
Límite:
Femenino
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Oncology
Año:
2002
Tipo del documento:
Artículo
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