cDNA array in the establishment of a gene expression profile associated with differentiation inducing the glioma cells / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 222-225, 2002.
Article
en Zh
| WPRIM
| ID: wpr-301969
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Establishment of a gene expression profile associated with differentiation inducing the glioma cells was made possible.</p><p><b>METHOD</b>The expression level of 18 000 genes in glioma cells was evaluated before and after induction with sodium phenyl-butyrate for 2 hours or 6 days by cDNA array technique, with the results proved by multi-dot blot.</p><p><b>RESULTS</b>Ninety-eight gene expressions in the glioma cells were changed after the induction, with some genes in transcription and translation systems down-regulated, some oncogenes down-regulated, and some differentiation or apoptosis genes up-regulated. Eighteen unknown EST fragments were changed also.</p><p><b>CONCLUSION</b>A gene expression profile associated with differentiation-inducing the glioma cells including 98 genes has been established.</p>
Texto completo:
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Índice:
WPRIM
Asunto principal:
Patología
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ADN de Neoplasias
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ARN Neoplásico
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Expresión Génica
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Diferenciación Celular
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Análisis de Secuencia por Matrices de Oligonucleótidos
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Perfilación de la Expresión Génica
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Genética
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Glioma
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Metabolismo
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Año:
2002
Tipo del documento:
Article