Effect of human calcyclin binding protein encoding gene on development of multiple drug resistance in gastric cancer / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 426-429, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-301996
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of human calcyclin binding protein (CacyBP) encoding gene on the development of multiple drug resistance in gastric cancer.</p><p><b>METHODS</b>hCacyBP sense nucleic acid eukaryotic expression vector (pcDNA3.1/hCacyBP +) was constructed and then transfected steadily into the gastric cancer drug sensitive cell (SGC7901) mediated by lipofectamine ( trade mark ) 2000. RT-PCR was used to measure the CacyBP mRNA expression level. MTT was used to measure the adriamycin (ADR) drug sensitivity of SGC7901 and SGC7901 after transfection. FCM was used to measure the average ADR accumulation concentration and cell cycle of SGC7901 and SGC7901 after transfection.</p><p><b>RESULTS</b>The hCacyBP mRNA expression level of SGC7901 transfected with pcDNA3.1/hCacyBP + was higher than SGC7901 transfected with pcDNA3.1 or SGC7901, with the higher survival rate in the former. The average ADR accumulation concentration in SGC7901 and SGC7901 transfected with pcDNA3.1 or pcDNA3.1/hCacyBP + was 5.64, 5.49 and 5.17, respectively. The G(1) phase cell proportion of SGC7901 transfected with pcDNA3.1/hCacyBP + or pcDNA3.1 was reduced slightly but G(2) and S phases increased slightly as compared with SGC7901.</p><p><b>CONCLUSION</b>Calcyclin binding protein may play a certain role in gastric cancer drug resistance.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Fisiología
/
Neoplasias Gástricas
/
Proteínas de Unión al Calcio
/
Ensayos de Selección de Medicamentos Antitumorales
/
Datos de Secuencia Molecular
/
Células Tumorales Cultivadas
/
Secuencia de Bases
/
Técnicas de Transferencia de Gen
/
ADN Complementario
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Oncology
Año:
2002
Tipo del documento:
Artículo
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