Effects of Bufalin on SYK and CBL family proteins in induction of HL-60 cell apoptosis / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 65-68, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-302196
ABSTRACT
The study was aimed to explore the mechanism of SYK and CBL family of ubiquitin ligases in Bufalin-induced HL-60 cells apoptosis. Cell viability was tested by trypan blue staining and apoptosis was detected by using flow cytometry. The expressions of CBL and CBL-b and the phosphorylation of SYK were detected by using immunoprecipitation and Western blot. The results showed that Bufalin inhibited HL-60 cell proliferation in time- and dose-dependent manners. IC(50) of suppressing cell viability at 24, 48 and 72 hours were about 26.3, 7.8 and 2.0 nmol/L respectively. The high dose of bufalin already induced apoptosis of HL-60 cells at 8 hours. SYK was quickly phosphorylated, and the expressions of CBL and CBL-b were down-regulated after treatment with Bufalin. It is concluded that SYK activation and CBL protein down-regulation may be involved in Bufalin-induced HL-60 cell apoptosis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Proteínas Tirosina Quinasas
/
Bufanólidos
/
Transducción de Señal
/
Regulación hacia Abajo
/
Regulación Leucémica de la Expresión Génica
/
Apoptosis
/
Células HL-60
/
Péptidos y Proteínas de Señalización Intracelular
/
Proliferación Celular
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2009
Tipo del documento:
Artículo
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