Prolongation of functional life-span of neutrophils by recombinant verotoxin 2 / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 900-903, 2002.
Artículo
en Inglés
| WPRIM
| ID: wpr-302278
ABSTRACT
<p><b>OBJECTIVE</b>Verotoxin-producing Escherichia coli (VTEC) strains of serotype O157 H7 have been implicated in a wide spectrum of diseases, including blood diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). To further explore the pathological role of verotoxin (VT) in HUS and other VTEC associated diseases, we investigated the effects of recombinant verotoxin 2 (rVT2) on the biological activity of neutrophils.</p><p><b>METHODS</b>The technique of flow cytometry, a fluorescent probe 2,7-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF/AM), and the assay of reduced cytochrome c to detect superoxide production were used in this study.</p><p><b>RESULTS</b>gammaVT2 significantly inhibited spontaneous apoptosis in neutrophils. Neutrophils with prolonged survival due to gammaVT2 maintained various biological functions, such as the expression of adhesion molecules (shading CD62L and raising CD11b/CD18), adherence to human umbilical vein endothelial cells (HUVECs), and generation of superoxide (O(2)(-)).</p><p><b>CONCLUSION</b>Prolongation of the functional life-span of neutrophils by gammaVT2 may accelerate inflammatory responses at sites of inflammation. This may play a crucial role in neutrophil-mediated tissue injury in HUS and other VTEC-associated diseases.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Proteínas Recombinantes
/
Endotelio Vascular
/
Adhesión Celular
/
Apoptosis
/
Superóxidos
/
Toxina Shiga II
/
Biología Celular
/
Toxicidad
/
Metabolismo
Límite:
Humanos
Idioma:
Inglés
Revista:
Chinese Medical Journal
Año:
2002
Tipo del documento:
Artículo
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