Inhibitory effect of pumpkin protein on expression of Notch signal in RPMI8226 myeloma cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1012-1015, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-302357
ABSTRACT
This study was aimed to explore the inhibitory effect of pumpkin protein (cucurmosin, CUS) on proliferation of RPMI8226 myeloma cells in vitro and its mechanism. Western blot was used to detect the expression level of Notch-1, Jagged-2, P-Akt and NF-KB in the myeloma cells treated by different concentrations of CUS. The results demonstrated that CUS could down-regulate the protein expression levels of Notch1, Jagged-2, P-Akt and NF-KB in the myeloma cells and with time-and concentration-dependent way, at the same time CUS could also decrease the expressions of BCL-2 and P-Akt. It is concluded that CUS can obviously inhibit the RPMI8226 cell proliferation in vitro, down-regulate the expression levels of Notch signal and its down-stream target genes. Therefore, Notch signaling pathway can be used as a new treatment target for multiple myeloma, and CUS may be become a potential new drug for regulating Notch signaling pathway.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Proteínas de Plantas
/
Transducción de Señal
/
FN-kappa B
/
Péptidos y Proteínas de Señalización Intercelular
/
Línea Celular Tumoral
/
Proteínas Proto-Oncogénicas c-akt
/
Receptores Notch
/
Receptor Notch1
/
Proteína Jagged-2
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2014
Tipo del documento:
Artículo
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