C-jun N-terminal kinase-mediated signaling is essential for Staphylococcus aureus-induced U937 apoptosis / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 26-29, 2009.
Artículo
en Inglés
| WPRIM
| ID: wpr-302655
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of SP600125, a specific c-jun N-terminal protein kinase (JNK) inhibitor, on Staphylococcus aureus (S. aureus)-induced U937 cell death and the underlying mechanism.</p><p><b>METHODS</b>The human monocytic U937 cells were treated with S. aureus at different time with or without SP600125. Cell apoptosis was analyzed by flow cytometry. JNK, Bax, and caspase-3 activities were detected by Western blotting.</p><p><b>RESULTS</b>S. aureus induced apoptosis in cultured U937 cells in a time-dependent manner. Expression of Bax and phospho-JNK significantly increased in S. aureus-treated U937 cells, and the level of activated caspase-3 also increased in a time-dependent manner. Inhibition of JNK with SP600125 significantly inhibited S. aureus-induced apoptosis in U937 cells.</p><p><b>CONCLUSIONS</b>S. aureus can induce apoptosis in U937 cells by phosphorylation of JNK and activation of Bax and caspase-3. SP600125 protects U937 cells from apoptosis induced by S. aureus via inhibiting the activity of JNK.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Fosforilación
/
Fisiología
/
Staphylococcus aureus
/
Transducción de Señal
/
Apoptosis
/
Células U937
/
Biología Celular
/
Proteínas Quinasas JNK Activadas por Mitógenos
/
Proteína Quinasa 8 Activada por Mitógenos
Límite:
Humanos
Idioma:
Inglés
Revista:
Chinese Medical Sciences Journal
Año:
2009
Tipo del documento:
Artículo
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