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Determination of clonidine in rabbit plasma by HPLC-MS / 药学学报
Acta Pharmaceutica Sinica ; (12): 367-369, 2004.
Artículo en Chino | WPRIM | ID: wpr-302807
ABSTRACT
<p><b>AIM</b>To determine clonidine in rabbit plasma by LC-MS.</p><p><b>METHODS</b>The LC-MS system consisted of Waters Alliance 2790 HPLC and Micromass ZQ-4000 MS. The HPLC was performed by using XTerra C18 (150 mm x 2.1 mm ID, 5 microm). The mobile phase, consisting of acetonitrile/ammonium hydrogen carbonate solution, was maintained to a flow-rate of 0.2 mL x min(-1) and the linear gradient elution was adopted. Mass spectrum was obtained by using electrospray ionization interface and the m/z of SIM was 230.</p><p><b>RESULTS</b>The average recovery was high and the method was reproducible. The calibration curve showed good linearity in the range of 1 - 80 microg x L(-1), the lowest limit of detection was 0.05 microg x L(-1). The Cmax, AUC0-t, and Tmax value of the pharmacokinetics parameter were (27 +/- 9) microg x L(-1), (5,352 +/- 1,121) microg x L(-1), (79 +/- 17) h.</p><p><b>CONCLUSION</b>The results demonstrated that the method had high sensitivity, good selectivity, accuracy and precision. It is used to determine the clonidine concentration in plasma. The transdermal patch can deliver clonidine to the surface of rabbit skin stably for periods of up to 1 week after a single application.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Sangre / Administración Cutánea / Farmacocinética / Cromatografía Líquida de Alta Presión / Clonidina / Área Bajo la Curva / Espectrometría de Masa por Ionización de Electrospray / Métodos / Antihipertensivos Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Chino Revista: Acta Pharmaceutica Sinica Año: 2004 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Sangre / Administración Cutánea / Farmacocinética / Cromatografía Líquida de Alta Presión / Clonidina / Área Bajo la Curva / Espectrometría de Masa por Ionización de Electrospray / Métodos / Antihipertensivos Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Chino Revista: Acta Pharmaceutica Sinica Año: 2004 Tipo del documento: Artículo