Survival properties of ETEC surface-displayed K88ac-LT(B) on Lactobacillus casei / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 43-48, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-302857
ABSTRACT
K88ac-LT(B) gene derived from pQE30-K88ac-LT(B) was cloned into the expression vector pLA and then the recombinant vector was transformed into the competent cells Lactobacillus casei 525. The recombinant bacteria were grown at 37 degrees C, in MRS broth. Western blotting analysis with rabbit-anti-K88ac-LT(B) polyclonal serum indicated that the recombinant protein reacted with the specific antibodies. The results showed that the molecular weight of the recombinant protein was about 71.2 kD. The K88ac-LT(B) fusion protein on the cell surface was confirmed by immunofluorescence mciroscopy and flow cytometric analysis. In addition, the survival of recombinant Lactobacillus casei 525 was studied in imitative gastrointestinal environments such as artificial gastro fluid (pH 1.5-5.5), artificial intestinal fluid, bile(0.3-3.0 g/L). The results indicated that the recombinant strain survived well in artificial gastric fluids at pH 2.5-4.5 in 5 h. The recombinant Lactobacillus casei 525 could slowly grow in the artificial intestinal fluid for different time, and could survive in 0.3% bile.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Recombinación Genética
/
Toxinas Bacterianas
/
Proteínas Recombinantes de Fusión
/
Proteínas de Escherichia coli
/
Proteínas Fimbrias
/
Enterotoxinas
/
Escherichia coli Enterotoxigénica
/
Jugo Gástrico
/
Genética
/
Lacticaseibacillus casei
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2009
Tipo del documento:
Artículo
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