Inhibitory effect of p53 gene on telomerase activity and proliferative activity in salivary adenoid cystic carcinoma cells / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 45-48, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-303437
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the inhibitory effect of p53 gene on salivary adenoid cystic carcinoma (SACC) cells.</p><p><b>METHODS</b>Adenoviral vector pDeltaE1-p53 was constructed and transfected into SACC-83 cells. The enhanced p53 expression was measured by reverse transcription polymerase chain reaction (RT-PCR), and the effects of transfected p53 on SACC-83 cells were analyzed by TRAP-PCR-ELISA, luciferase reporter, flow cytometry (FCM), soft agar assay and tumorigenicity test.</p><p><b>RESULTS</b>The expression of p53 gene in SACC-83 cells was increased after introduction of pDeltaE1-p53. The telomerase activity and the transcriptional activity of hTERT promoter were inhibited. The cells cycles of transfected SACC-83 were arrested in G(1) phase and the rate of colony-formation was decreased, and similarly the tumorigenicity in nude mice was also decreased.</p><p><b>CONCLUSIONS</b>The introduction of wild-type p53 by adenoviral vector could suppress the telomerase activity and malignant phenotypes of salivary adenoid cystic carcinoma cells.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Neoplasias de las Glándulas Salivales
/
Transfección
/
Proteína p53 Supresora de Tumor
/
Carcinoma Adenoide Quístico
/
Telomerasa
/
Línea Celular Tumoral
/
Vectores Genéticos
/
Genética
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Stomatology
Año:
2006
Tipo del documento:
Artículo
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