In vitro inhibition activity of polyphenol-rich extracts from Syzygium aromaticum (L.) Merr. & Perry (Clove) buds against carbohydrate hydrolyzing enzymes linked to type 2 diabetes and Fe(2+)-induced lipid peroxidation in rat pancreas
Asian Pacific Journal of Tropical Biomedicine
; (12): 774-781, 2012.
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| ID: wpr-303602
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ABSTRACT
<p><b>OBJECTIVE</b>To investigate and compare the inhibitory properties of free and bound phenolic extracts of clove bud against carbohydrate hydrolyzing enzymes (alpha-amylase & alpha-glucosidase) and Fe(2+)-induced lipid peroxidation in rat pancreas in vitro.</p><p><b>METHODS</b>The free phenolics were extracted with 80% (v/v) acetone, while bound phenolics were extracted from the alkaline and acid hydrolyzed residue with ethyl acetate. Then, the interaction of the extracts with alpha-amylase and alpha-glucosidase was subsequently assessed. Thereafter, the total phenolic contents and antioxidant activities of the extracts were determined.</p><p><b>RESULTS</b>The result revealed that both extracts inhibited alpha-amylase and alpha-glucosidase in a dose-dependent manner. However, the alpha-glucosidase inhibitory activity of the extracts were significantly (P<0.05) higher than their alpha-amylase inhibitory activity. The free phenolics (31.67 mg/g) and flavonoid (17.28 mg/g) contents were significantly (P<0.05) higher than bound phenolic (23.52 mg/g) and flavonoid (13.70 mg/g) contents. Both extracts also exhibited high antioxidant activities as typified by their high reducing power, 1,1 diphenyl-2- picrylhydrazyl (DPPH) and 2, 2-azinobis-3-ethylbenzo-thiazoline-6-sulfonate (ABTS) radical scavenging abilities, as well as inhibition of Fe(2+)-induced lipid peroxidation in rat pancreas in vitro.</p><p><b>CONCLUSIONS</b>This study provides a biochemical rationale by which clove elicits therapeutic effect on type 2 diabetes.</p>
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Asunto principal:
Páncreas
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Farmacología
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Fenoles
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Flavonoides
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Extractos Vegetales
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Compuestos Ferrosos
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Peroxidación de Lípido
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Química
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Concentración 50 Inhibidora
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Syzygium
Límite:
Animals
Idioma:
En
Revista:
Asian Pacific Journal of Tropical Biomedicine
Año:
2012
Tipo del documento:
Article