Preliminary study on effect of ganhuang injection on reject reaction of xenograft / 中国中西医结合杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of Ganhuang Injection (GHI) on reject reaction of xenograft.</p><p><b>METHODS</b>RT-PCR technique was used to detect the activated relevant gene expression in porcine endothelial cells (PEC), and 51Cr releasing method was used to test the killing and adhesion action of NK cells.</p><p><b>RESULTS</b>The normal human serum and human NK-92 cell could up-regulate the mRNA expressions of E-selectin and IL-1 alpha gene in PEC, showing the PEC activating action, GHI could inhibit these activated gene expressions. Cyto-toxic experiment showed that GHI could also inhibit the cytotoxicity of NK cell on PEC dose-dependently, which was in accord with its inhibition on adhesive action of NK on PEC.</p><p><b>CONCLUSION</b>GHI could inhibit not only the PEC activation in hyperacute rejection, but also the function of NK cells in delayed xenograft rejection.</p>